The Toxoplasma secreted effector TgWIP modulates dendritic cell motility by activating host tyrosine phosphatases Shp1 and Shp2.
Pavel Morales, Abbigale J. Brown, Lamba Omar Sangaré, Sheng Yang, Simon V.N.P. Kuihon, Baoyu Chen, and Jeroen P.J. Saeij
Cellular and Molecular Life Sciences​. 81, 294. (2024) (​PMID: 38977495)
Synthetic control of actin polymerization and symmetry breaking in active protocells.
​Shiva Razavi, Felix Wong, Bedri Abubaker-Sharif, Hideaki T. Matsubayashi, Hideki Nakamura, Nhung Thi Hong Nguyen, Douglas N. Robinson, Baoyu Chen, Pablo A. Iglesias, and Takanari Inoue.  
Science Advances. 10 (24) eadk9731. (2024) (​PMID: 38865458)
Structural basis for Retriever-SNX17 assembly and endosomal sorting.
Amika Singla*, Daniel J. Boesch*, Ho Yee Joyce Fung*, Chigozie Ngoka, Avery S. Enriquez, Ran Song, Daniel A. Kramer, Yan Han, Puneet Juneja, Daniel D. Billadeau, Xiaochen Bai, Zhe Chen, Emre E. Turer#, Ezra Burstein#, and Baoyu Chen#.
​BioRxiv. 584676 [Preprint]. March 13, 2024. ​
The NADPH oxidase 2 subunit p47phox binds to the WAVE regulatory complex and p22phox in a mutually exclusive manner. ​
Simon V.N.P. Kuihon, Brodrick J. Sevart, Colette A. Abbey, Kayla J. Bayless, and Baoyu Chen.
Journal of Biological Chemistry. 300(4):107130. (2024) (PMID: 38432630)​
Structural organization of the Retriever-CCC endosomal recycling complex. ​
Daniel J. Boesch*, Amika Singla*, Yan Han*, Daniel A. Kramer, Qi Liu, Kohei Suzuki, Puneet Juneja, Xuefeng Zhao, Xin Long, Michael J. Medlyn, Daniel D. Billadeau, Zhe Chen#, Baoyu Chen#, and Ezra Burstein#.
Nature Structural & Molecular Biology. (2023) (PMID: 37397996​)​
PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex.​
Yanan Wang, Giovanni Chiappetta*, Raphaël Guérois*, Yijun Liu*, Stéphane Romero*, Daniel J. Boesch, Matthias Krause, Claire Dessalles, Avin Babataheri, Abdul I. Barakat, Baoyu Chen, Joelle Vinh, Anna Polesskaya, and Alexis M. Gautreau.
Nature Communications. 14, 3541. (2023) (PMID: 37322026)
The intrinsically disordered cytoplasmic tail of a dendrite branching receptor uses two distinct mechanisms to regulate the actin cytoskeleton.
Daniel A. Kramer, Heidy Y. Narvaez-Ortiz, Urval Patel, Rebecca Shi, Kang Shen, Brad J. Nolen,  Julien Roche#, and Baoyu Chen#.  
eLife. 2:e88492 (2023) (PMID: 37555826)​
Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control.
Sabrina Schlienger,  Patricia T. Yam,  Nursen Balekoglu, Hugo Ducuing,  Jean-Francois Michaud, Shirin Makihara, Daniel A. Kramer, Baoyu Chen, Alfonso Fasano,  Alfredo Berardelli, Fadi F. Hamdan, Guy A. Rouleau, Myriam Srour,  and Frederic Charron. 
Science Advances. 19 (9) add5501. (2023) (PMID: 37172092)
Arf GTPase activates the WAVE Regulatory Complex through a distinct binding site.
Sheng Yang, Yubo Tang*, Yijun Liu*, Abbigale J. Brown, Matthias Schaks, Bojian Ding, Daniel A. Kramer, Magdalena Mietkowska, Li Ding, Olga Alekhina, Daniel D. Billadeau, Saikat Chowdhury, Junmei Wang, Klemens Rottner#, and Baoyu Chen#. 
Science Advances. 50 (8) add1412. (2022) (PMID: 36516255​)
Structures reveal a key mechanism of WAVE Regulatory Complex activation by Rac1 GTPase.
​Bojian Ding*, Sheng Yang*, Matthias Schaks, Yijun Liu, Abbigale J. Brown,  Klemens Rottner, Saikat Chowdhury#, and Baoyu Chen#. 
Nature Communications. 13, 5444. (2022) (PMID: 36114192)
A human DCC variant causing mirror movement disorder reveals an essential role for the Wave regulatory complex in Netrin/DCC signaling.
Karina Chaudhari, Kaiyue Zhang, Patricia T. Yam, Yixin Zang, Daniel A. Kramer, Sabrina Schlienger, Sara Calabretta, Myriam Srour, Baoyu Chen, Frédéric Charron, and Greg J. Bashaw. 
BioRxiv. 511954 [Preprint]. October 17, 2022.
CYFIP2-containing WAVE complexes inhibit cell migration by a competition mechanism.
Anna Polesskaya, Arthur Boutillon, Sheng Yang, Yanan Wang, Stéphane Romero, Yijun Liu, Marc Lavielle, Sophie Vacher, Anne Schnitzler, Nicolas Molinie, Nathalie Rocques, Artem Fokin, Raphaël Guérois, Ivan Bièche, Baoyu Chen, Nicolas B. David, and Alexis M. Gautreau. 
BioRxiv. 184655 [Preprint]. August 21, 2021.
WASP family proteins: molecular mechanisms and implications in human disease. 
Daniel A. Kramer, Hannah K. Piper, and Baoyu Chen. 
European Journal of Cell Biology. 101 (3) 151244. (2022) (PMID: 35667337)​​
A two-step actin polymerization mechanism drives dendrite branching. 
Rebecca Shi, Daniel A. Kramer, Baoyu Chen, and Kang Shen. 
Neural Development. 16(1):3. (2021) (PMID: 34281597​)​​
WAVE regulatory complex. 
Klemens Rottner#, Theresia E.B. Stradal, and Baoyu Chen#. ​
Current Biology. 31 (10), R512-517. (2021) (PMID: 34033782​)​​
HEM1 deficiency disrupts mTORC2 and F-actin control in inherited immunodysregulatory disease. ​
Sarah A. Cook, William A. Comrie, M. Cecilia Poli, Morgan Similuk, Andrew J. Oler, Aiman J. Faruqi, Douglas B. Kuhns, Sheng Yang, Alexander Vargas-Hernández, Alexandre F. Carisey, Benjamin Fournier, D. Eric Anderson, Susan Price, Margery Smelkinson, Wadih Abou Chahla, Lisa R. Forbes, Emily M. Mace, Tram N. Cao, Zeynep H. Coban-Akdemir, Shalini N. Jhangiani, Donna M. Muzny, Richard A. Gibbs, James R. Lupski, Jordan S. Orange, Geoffrey D. E. Cuvelier, Moza Al Hassani, Nawal Al Kaabi, Zain Al Yafei, Soma Jyonouchi, Nikita Raje, Jason W. Caldwell, Yanping Huang, Janis K. Burkhardt, Sylvain Latour, Baoyu Chen, Gehad ElGhazali, V. Koneti Rao, Ivan K. Chinn, and Michael J. Lenardo. 
Science. 369 (6500): 202-207. (2020) (PMID: 32647003)
Endosomal Receptor Trafficking: Retromer and Beyond. ​
Jing Wang, Alina Fedoseienko, Baoyu Chen, Ezra Burstein, Da Jia, and Daniel D. Billadeau. 
Traffic. 19(8):​ 578-590. (2018) (PMID: 29667289)
A Dendritic Guidance Receptor Complex Brings Together Distinct Actin Regulators to Drive Efficient F-Actin Assembly and Branching.
Wei Zou#, Xintong Dong, Timothy R. Broederdorf, Ao Shen, Daniel A. Kramer, Rebecca Shi, Xing Liang, David M. Miller III, Yang K. Xiang, Ryohei Yasuda, Baoyu Chen#, and Kang Shen#.  ​
Developmental Cell. 45 (3) 362–375.e3 (2018) (PMID: 29738713​)
Rac1 GTPase activates the WAVE regulatory complex through two distinct binding sites. 
Baoyu Chen#, Hui-Ting Chou, Chad A Brautigam, Wenmin Xing, Sheng Yang, Lisa Henry, Lynda K Doolittle, Thomas Walz, and Michael K Rosen#. 
eLife. 6:e29795 (2017) (PMID: 28949297​)​
​​Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation. 
Anna Julia Squarr, Klaus Brinkmann, Baoyu Chen, Tim Steinbacher, Klaus Ebnet, Michael K. Rosen, and Sven Bogdan. 
Journal of Cell Biology. 212 (5), 591-603. (2016) (PMID: 26903538​)
Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton. 
Xing Judy Chen, Anna Julia Squarr*, Raiko Stephan*, Baoyu Chen*, Theresa E. Higgins, David J. Barry, Morag C. Martin, Michael K. Rosen, Sven Bogdan, and Michael Way. 
​Developmental Cell. 30 (5), 569-584. (2014) (PMID: 25203209)
Biochemical reconstitution of the WAVE regulatory complex.
Baoyu Chen, Shae B Padrick, Lisa Henry, and Michael K. Rosen.  
​Methods in Enzymology. 540: 55-72. (2014) (PMID: 24630101)
The WAVE regulatory complex links diverse receptors to the actin cytoskeleton.
Baoyu Chen, Klaus Brinkmann, Zhucheng Chen, Chi W. Pak, Yuxing Liao, Shuoyong Shi, Lisa Henry, Nick Grishin, Sven Bogdan, and Michael K. Rosen. 
​Cell. 156 (1-2), 195-207. (2014) (PMID: 24439376​)
Local F-actin network links synapse formation and axon branching. 
Poh Hui Chia, Baoyu Chen, Pengpeng Li, Michael K. Rosen, and Kang Shen. 
​Cell. 156 (1-2), 208-220. (2014) (PMID: 24439377​)​
Phase transitions in the assembly of multivalent signaling proteins. 
Pilong Li, Sudeep Banjade, Hui-Chun Cheng, Soyeon Kim, Baoyu Chen, Liang Guo, Marc LIaguno, Javoris V. Hollingsworth, David S. King, Salman F. Banani, Paul S. Russo, Qiu-Xing Jiang, B. Tracy Nixon, and Michael K. Rosen. 
​Nature. 483 (7389), 336-340. (2012) (PMID: 22398450)​
Engagement of arginine finger to ATP triggers large conformational changes in NtrC1 AAA+ ATPase for remodeling bacterial RNA polymerase. 
Baoyu Chen*, Tatyana A. Syseova*, Saikat Chowdhury, Liang Guo, Sacha De Carlo, Jeffrey A. Hansen, Haw Yang, and B. Tracy Nixon. 
​Structure. 18(11), 1420-1430. (2010)
The WAVE regulatory complex is inhibited. 
Ayman M. Ismail, Shae B. Padrick, Baoyu Chen, Junko Umetani, and Michael K Rosen. 
​Nature Structural & Molecular Biology. 16(5): 561-563. (2009) (PMID: 19363480)​
 ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by copurification of adenylate kinase of Escherichia coli.
Baoyu Chen, Tatyana A. Syseova, Saikat Chowdhury, Liang Guo, and B. Tracy Nixon.  
​FEBS Journal. 276(3): 807-815. (2009)
Regulation and action of the bacterial enhancer protein AAA+ ATPases. 
Baoyu Chen, Tatyana A. Syseova, Saikat Chowdhury, and B. Tracy Nixon. 
​Biochemical Society Transactions. 36(Pt 1): 89-93. (2008)
ATP ground- and transition states of bacterial enhancer binding AAA+ ATPases support complex formation with their target protein, s54. 
Baoyu Chen, Michaeleen Doucleff, David E. Wemmer, Sacha De Carlo, Hector H. Huang, Eva Nogales, Timothy R. Hoover, Elena Kondrashkina, Liang Guo, and B. Tracy Nixon. 
​Structure. 15(4): 429-220. (2007)
The structural basis for regulated assembly and function of the transcriptional activator NtrC. 
Sacha De Carlo*, Baoyu Chen*, Timothy R. Hoover, Elena Kondrashkina, Eva Nogales, and B. Tracy Nixon. 
​Genes & Development. 20: 1485-1495. (2006)
Negative regulation of AAA+ ATPase assembly by two component receiver domains: a transcription activation mechanism that is conserved in mesophilic and extremely hyperthermophilic bacteria. 
Michaeleen Doucleff, Baoyu Chen, Ann E. Maris, David E. Wemmer, Elena Kondrashkina, and B. Tracy Nixon. 
​Journal of Molecular Biology. 353: 242-255. (2005)
Evidence for proximal cysteine and lysine residues at or near the active site of arginine kinase of Stichopus japonicus. 
Qin Guo, Baoyu Chen and Xicheng Wang. 
​Biochemistry (Moscow). 69:1336-1343. (2004)
Expression, purification, and characterization of arginine kinase from the sea cucumber Stichopus japonicus.
Suyuan Guo, Zhi Guo, Qin Guo, Baoyu Chen, and Xicheng Wang.  
​Protein Expression & Purification. 29: 230-234. (2003)
​An improved activity assay method for arginine kinase based on a ternary heteropolyacid system.
Baoyu Chen, Qin Guo, Zhi Guo, and Xicheng Wang.
Tsinghua Science and Technology. 8: 422-427. (2003) 
Roy J. Carver Department of Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, Iowa, 50014.
​© COPYRIGHT 2016. ALL RIGHTS RESERVED.