Publications

Daniel J. Boesch*, Amika Singla*, Yan Han*, Daniel A. Kramer, Qi Liu, Kohei Suzuki, Puneet Juneja, Xuefeng Zhao, Xin Long, Michael J. Medlyn, Daniel D. Billadeau, Zhe Chen#, Baoyu Chen#, Ezra Burstein#. Structural organization of the Retriever-CCC endosomal recycling complex. Nature Structural & Molecular Biology (in press) [Preprint]

Shiva Razavi, Felix Wong, Bedri Abubaker-Sharif, Hideaki T. Matsubayashi, Hideki Nakamura, Eduardo Sandoval, Douglas N. Robinson, Baoyu Chen, Jian Liu, Pablo A. Iglesias, Takanari Inoue. Synthetic control of actin polymerization and symmetry breaking in active protocells. BioRxiv 559060 [Preprint]. September 23, 2023.

Yanan Wang, Giovanni Chiappetta*, Raphaël Guérois*, Yijun Liu*, Stéphane Romero*, Daniel J. Boesch, Matthias Krause, Claire Dessalles, Avin Babataheri, Abdul I. Barakat, Baoyu Chen, Joelle Vinh, Anna Polesskaya, Alexis M. Gautreau. PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex. Nature Communications. 14, 3541. (2023)

Daniel A. Kramer, Heidy Y. Narvaez-Ortiz, Urval Patel, Rebecca Shi, Kang Shen, Brad J. Nolen, Julien Roche#, Baoyu Chen#. The intrinsically disordered cytoplasmic tail of a dendrite branching receptor uses two distinct mechanisms to regulate the actin cytoskeleton. eLife. 12:e88492 (2023)

Sabrina Schlienger, Patricia T. Yam, Nursen Balekoglu, Hugo Ducuing, Jean-Francois Michaud, Shirin Makihara, Daniel A. Kramer, Baoyu Chen, Alfonso Fasano, Alfredo Berardelli, Fadi F. Hamdan, Guy A. Rouleau, Myriam Srour, Frederic Charron. Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control. Science Advances. 19 (9) add5501. (2023)

Sheng Yang, Yubo Tang*, Yijun Liu*, Abbigale J. Brown, Matthias Schaks, Bojian Ding, Daniel A. Kramer, Magdalena Mietkowska, Li Ding, Olga Alekhina, Daniel D. Billadeau, Saikat Chowdhury, Junmei Wang, Klemens Rottner# and Baoyu Chen#. Arf GTPase activates the WAVE Regulatory Complex through a distinct binding site. Science Advances. 50 (8) add1412. (2022) (PDF)
- On the news: English; Deutsch; 中文

Bojian Ding*, Sheng Yang*, Matthias Schaks, Yijun Liu, Abbigale J. Brown, Klemens Rottner, Saikat Chowdhury# and Baoyu Chen#. Structures reveal a key mechanism of WAVE Regulatory Complex activation by Rac1 GTPase. Nature Communications. 13, 5444. (2022) (PDF)

Karina Chaudhari, Kaiyue Zhang, Patricia T. Yam, Yixin Zang, Daniel A. Kramer, Sabrina Schlienger, Sara Calabretta, Myriam Srour, Baoyu Chen, Frédéric Charron and Greg J. Bashaw. A human DCC variant causing mirror movement disorder reveals an essential role for the Wave regulatory complex in Netrin/DCC signaling. BioRxiv 511954 [Preprint]. October 17, 2022.

Anna Polesskaya, Arthur Boutillon, Sheng Yang, Yanan Wang, Stéphane Romero, Yijun Liu, Marc Lavielle, Sophie Vacher, Anne Schnitzler, Nicolas Molinie, Nathalie Rocques, Artem Fokin, Raphaël Guérois, Ivan Bièche, Baoyu Chen, Nicolas B. David, Alexis M. Gautreau. CYFIP2-containing WAVE complexes inhibit cell migration by a competition mechanism. BioRxiv 184655 [Preprint]. August 21, 2021.

Daniel A. Kramer, Hannah K. Piper, Baoyu Chen. WASP family proteins: molecular mechanisms and implications in human disease. European Journal of Cell Biology. 101 (3) 151244. (2022) (PDF)

Rebecca Shi, Daniel A. Kramer, Baoyu Chen, Kang Shen. A two-step actin polymerization mechanism drives dendrite branching. Neural Development. 16(1):3. (2021) (PDF)

Klemens Rottner#, Theresia E.B. Stradal, Baoyu Chen#. WAVE regulatory complex. Current Biology. 31 (10), R512-517. (2021) (PDF)

Sarah A. Cook, William A. Comrie, M. Cecilia Poli, Morgan Similuk, Andrew J. Oler, Aiman J. Faruqi, Douglas B. Kuhns, Sheng Yang, Alexander Vargas-Hernández, Alexandre F. Carisey, Benjamin Fournier, D. Eric Anderson, Susan Price, Margery Smelkinson, Wadih Abou Chahla, Lisa R. Forbes, Emily M. Mace, Tram N. Cao, Zeynep H. Coban-Akdemir, Shalini N. Jhangiani, Donna M. Muzny, Richard A. Gibbs, James R. Lupski, Jordan S. Orange, Geoffrey D. E. Cuvelier, Moza Al Hassani, Nawal Al Kaabi, Zain Al Yafei, Soma Jyonouchi, Nikita Raje, Jason W. Caldwell, Yanping Huang, Janis K. Burkhardt, Sylvain Latour, Baoyu Chen, Gehad ElGhazali, V. Koneti Rao, Ivan K. Chinn, Michael J. Lenardo. HEM1 deficiency disrupts mTORC2 and F-actin control in inherited immunodysregulatory disease. Science. 369 (6500): 202-207. (2020) (PDF)

Jing Wang, Alina Fedoseienko, Baoyu Chen, Ezra Burstein, Da Jia, Daniel D. Billadeau. Endosomal Receptor Trafficking: Retromer and Beyond. Traffic. 19(8): 578-590. (2018) (PDF)

Wei Zou#, Xintong Dong, Timothy R. Broederdorf, Ao Shen, Daniel A. Kramer, Rebecca Shi, Xing Liang, David M. Miller III, Yang K. Xiang, Ryohei Yasuda, Baoyu Chen#, and Kang Shen#. A Dendritic Guidance Receptor Complex Brings Together Distinct Actin Regulators to Drive Efficient F-Actin Assembly and Branching. Developmental Cell. 45 (3) 362–375.e3 (2018) (PDF)
- On the news: 中文

Baoyu Chen#, Hui-Ting Chou, Chad A Brautigam, Wenmin Xing, Sheng Yang, Lisa Henry, Lynda K Doolittle, Thomas Walz and Michael K Rosen#. Rac1 GTPase activates the WAVE regulatory complex through two distinct binding sites. eLife. 6:e29795 (2017) (PDF)
- Faculty Opinions

Anna Julia Squarr, Klaus Brinkmann, Baoyu Chen, Tim Steinbacher, Klaus Ebnet, Michael K. Rosen and Sven Bogdan. Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation. Journal of Cell Biology. 212 (5), 591-603. (2016) (PDF)
- Highlighted in Journal of Cell Biology. 212 (5), 487-489 “Comment”

Xing Judy Chen, Anna Julia Squarr*, Raiko Stephan*, Baoyu Chen*, Theresa E. Higgins, David J. Barry, Morag C. Martin, Michael K. Rosen, Sven Bogdan and Michael Way. Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton. Developmental Cell. 30 (5), 569-584. (2014) (PDF)

Baoyu Chen, Shae B Padrick, Lisa Henry, and Michael K. Rosen. Biochemical reconstitution of the WAVE regulatory complex. Methods in Enzymology. 540: 55-72. (2014) (PDF)

Baoyu Chen, Klaus Brinkmann, Zhucheng Chen, Chi W. Pak, Yuxing Liao, Shuoyong Shi, Lisa Henry, Nick Grishin, Sven Bogdan, and Michael K. Rosen. The WAVE regulatory complex links diverse receptors to the actin cytoskeleton. Cell. 156 (1-2), 195-207. (2014) (PDF)
- Highlighted in Cell, 156 (1-2), 13-4 “Preview”
- Recommended by Faculty of 1000

Poh Hui Chia, Baoyu Chen, Pengpeng Li, Michael K. Rosen, and Kang Shen. Local F-actin network links synapse formation and axon branching. Cell. 156 (1-2), 208-220. (2014) (PDF)
- Highlighted in Cell, 156 (1-2), 13-4 “Preview”

Pilong Li, Sudeep Banjade, Hui-Chun Cheng, Soyeon Kim, Baoyu Chen, Liang Guo, Marc LIaguno, Javoris V. Hollingsworth, David S. King, Salman F. Banani, Paul S. Russo, Qiu-Xing Jiang, B. Tracy Nixon, and Michael K. Rosen. Phase transitions in the assembly of multivalent signaling proteins. Nature. 483 (7389), 336-340. (2012) (PDF)
- Recommended by Faculty of 1000

Baoyu Chen*, Tatyana A. Syseova*, Saikat Chowdhury, Liang Guo, Sacha De Carlo, Jeffrey A. Hansen, Haw Yang and B. Tracy Nixon. Engagement of arginine finger to ATP triggers large conformational changes in NtrC1 AAA+ ATPase for remodeling bacterial RNA polymerase. Structure. 18(11), 1420-1430. (2010) (PDF)
- Highlighted in Structure, 18 (11), 1391-1392 “Preview”

Ayman M. Ismail, Shae B. Padrick, Baoyu Chen, Junko Umetani and Michael K Rosen. The WAVE regulatory complex is inhibited. Nature Structural and Molecular Biology. 16(5): 561-563. (2009) (PDF)
- Recommended by Faculty of 1000

Baoyu Chen, Tatyana A. Syseova, Saikat Chowdhury, Liang Guo, and B. Tracy Nixon. ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by copurification of adenylate kinase of Escherichia coli. FEBS Journal. 276(3): 807-815. (2009) (PDF)

Baoyu Chen, Tatyana A. Syseova, Saikat Chowdhury, and B. Tracy Nixon. Regulation and action of the bacterial enhancer protein AAA+ ATPases. Biochemical Society Transactions. 36(Pt 1): 89-93. (2008) (PDF)

Baoyu Chen, Michaeleen Doucleff, David E. Wemmer, Sacha De Carlo, Hector H. Huang, Eva Nogales, Timothy R. Hoover, Elena Kondrashkina, Liang Guo and B. Tracy Nixon. ATP ground- and transition states of bacterial enhancer binding AAA+ ATPases support complex formation with their target protein, s54. Structure. 15(4): 429-220. (2007) (PDF)

Sacha De Carlo*, Baoyu Chen*, Timothy R. Hoover, Elena Kondrashkina, Eva Nogales, and B. Tracy Nixon. The structural basis for regulated assembly and function of the transcriptional activator NtrC. Genes & Development. 20: 1485-1495. (2006) (PDF)

Michaeleen Doucleff, Baoyu Chen, Ann E. Maris, David E. Wemmer, Elena Kondrashkina and B. Tracy Nixon. Negative regulation of AAA+ ATPase assembly by two component receiver domains: a transcription activation mechanism that is conserved in mesophilic and extremely hyperthermophilic bacteria. Journal of Molecular Biology. 353: 242-255. (2005) (PDF)

Qin Guo, Baoyu Chen and Xicheng Wang. Evidence for proximal cysteine and lysine residues at or near the active site of arginine kinase of Stichopus japonicus. Biochemistry (Moscow). 69:1336-1343. (2004)

Suyuan Guo, Zhi Guo, Qin Guo, Baoyu Chen and Xicheng Wang. Expression, purification, and characterization of arginine kinase from the sea cucumber Stichopus japonicus. Protein Expression & Purification. 29: 230-234. (2003)

Baoyu Chen, Qin Guo, Zhi Guo and Xicheng Wang. An improved activity assay method for arginine kinase based on a ternary heteropolyacid system. Tsinghua Science and Technology. 8: 422-427. (2003)